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Increased Serum HMGB-1, ICAM-1 and Metalloproteinase-9 Levels in Buerger’s Patients

[ Vol. 14 , Issue. 4 ]

Author(s):

Giovanni De Caridi, Alessandra Bitto, Mafalda Massara, Giovanni Pallio, Gabriele Pizzino, Raffaele Serra, Domenica Altavilla, Francesco Squadrito and Francesco Spinelli   Pages 382 - 387 ( 6 )

Abstract:


Background: Thromboangiitis obliterans (TAO), or Buerger’s disease, is an inflammatory occlusive disorder that affects the limb arteries of young smokers. In the aetiology of TAO the immune system appears to play a critical role; however, information on the aspects involved in the evolution of vascular tissue inflammation and of this disease are still limited.

Objective: This study was carried out to investigate HMGB-1 (high mobility group box-1), MMP (matrix metalloproteinase)- 2, MMP-9, MMP-11 and ICAM (intercellular adhesion molecule)-1 circulating levels in subjects with Buerger’s disease.

Methods: Between January 2010 and December 2012, eight patients underwent surgical revascularization of the lower limbs and a specimen of the affected arterial wall was obtained for histological confirmation of Buerger’s disease. A blood sample was collected on the same day for measuring HMGB-1, MMP-3, MMP-9 and ICAM-1 by western blot analysis. Controls (n=7) were healthy non-smokers.

Results: TAO subjects had a significant increase in HMGB-1, MMP-9 and ICAM-1 compared with controls (P<.0001), while no differences were observed in MMP-2 and MMP-11 levels. Histology confirmed a strong inflammatory infiltrate with signs of necrosis in the arterial wall.

Conclusion: These data suggest a role for HMGB -1 in the vascular lesions associated with TAO, unveiling HMGB-1 as a potential target for treating this rare disease.

Keywords:

Buerger’s disease, MMPs, HMGB-1, surgical revascularization.

Affiliation:

Department of Clinical and Experimental Medicine, Section of Pharmacology, Torre Biologica 5th floor, c/o AOU “G. Martino”, Via C. Valeria, 98125, Messina, ITALY



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